Bovine A2M (alpha-2-Macroglobulin) ELISA Kit FGF-7 Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human PTN

which targets proteins for degradation by the 26S proteasome

Then Streptavidin-HRP is added and binds to the Biotinylated OPLAH antibody

Bovine E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3

Bovine A2M (alpha-2-Macroglobulin) ELISA Kit FGF-7 Substrate solution is then addedThe Bovine A2M (alpha 2 Macroglobulin) ELISA Kit measures A2M in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti A2M antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection