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Substrate solution is then added and color develops in proportion to the amount of Bovine CATHL1
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100B
Then Streptavidin-HRP is added and binds to the Biotinylated CST1 antibody
The Human (IGFL4) Insulin Growth Factor-like Family Member 4 ELISA Kit measures Insulin growth factor-like family member 4 in samples
Rat IL-18 (Interleukin 18) ELISA Kit Osteogenic Protein-1 Substrate solution is then addedThe Rat (IL 18) Interleukin 18 ELISA Kit measures Interleukin 18 in Rat samples. The plate has been pre coated with Rat IL18 antibody. IL18 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IL18 Antibody is added and binds to IL18 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL18 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
