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Substrate solution is then added and color develops in proportion to the amount of Porcine SP-B
And then biotinylated Human HACE1 Antibody is added and binds to HACE1 in the sample
The plate has been pre-coated with Mouse UCN antibody
Then Streptavidin-HRP is added and binds to the Biotinylated GAD2 antibody
Rat CKLF (Chemokine Like Factor) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat CKLF (Chemokine Like Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat CKLF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKLF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
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