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The concentration of Rat IL37 in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Human LL-37
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 40 nm
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CCR2
Mouse EFNB2 (Ephrin B2) ELISA Kit (Small Sample) TIMP3 The concentration of Rat IL37The Mouse EFNB2 (Ephrin B2) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse EFNB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse EFNB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
