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Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of RLN in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A10
Then a biotinylated detection antibody specific for Human NRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of SOD in the sample by plotting standard curve
Mouse CRH (Corticoliberin) ELISA Kit TIM Compare OD450 value of sampleThe Mouse CRH (Corticoliberin) ELISA Kit measures Crh in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Crh. Crh in the sample or standard competes with a fixed amount of biotin labeled Crh for sites on a pre coated antibody specific to Crh. Free components are washed off. After adding HRP
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