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Substrate solution is then added and color develops in proportion to the amount of Bovine BOLA3
The plate has been pre-coated with Rat HPSE antibody
You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Sheep LEP antibody
Human SDC4 (Syndecan 4) ELISA Kit GHIH Substrate solution is then addedThe Human (SDC4) Syndecan 4 ELISA Kit measures Syndecan 4 in samples. The plate has been pre coated with Human SDC4 antibody. SDC4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SDC4 Antibody is added and binds to SDC4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SDC4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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