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The OD value is proportional to the concentration of Human EGF
Then a biotinylated detection antibody specific for Human IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Wash unbound components again and add TMB substrate solution
The Mouse (RNLS) Renalase ELISA Kit measures Renalase in Mouse samples
Rat ROCK1 (Rho-associated Protein Kinase 1) ELISA Kit HAVCR The OD value is proportionalThe Rat (ROCK1) Rho associated Protein Kinase 1 ELISA Kit measures Rho Associated Protein Kinase 1 in Rat samples. The plate has been pre coated with Rat ROCK1 antibody. ROCK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ROCK1 Antibody is added and binds to ROCK1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ROCK1 antibody. After incubation unbound Streptavidin HRP
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