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The concentration of Horse TNFa in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Rat NFKB-p65
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AIRE
and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately
Human ABCB11 (Bile Salt Export Pump) ELISA Kit ACEII The concentration of Horse TNFaThe Human (ABCB11) Bile Salt Export Pump ELISA Kit measures Bile salt export pump in samples. The plate has been pre coated with Human ABCB11 antibody. ABCB11 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ABCB11 Antibody is added and binds to ABCB11 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ABCB11 antibody. After incubation unbound Streptavidin HRP is washed
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