Rat TNF-alpha (Tumor Necrosis Factor Alpha) ELISA Kit Nucleic Acid Purification and the Human VCAM1 standard

and the Human VCAM1 standard plate wells that pre-coated using protein-related techniques are provided separately

coli expression system and the target gene encoding Thr52-Ala118 is expressed

then added a HRP-conjugated antibody specific to Human IL-5

You can calculate the concentration of Mouse CKMB in the samples by comparing the OD of the samples to the standard curve

Rat TNF-alpha (Tumor Necrosis Factor Alpha) ELISA Kit Nucleic Acid Purification and the Human VCAM1 standardThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat TNF alpha. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat TNF alpha. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is