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Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light
The plate has been pre-coated with Human CASP14 antibody
And then biotinylated Mouse A1BG Antibody is added and binds to A1BG in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated KRT77 antibody
Human GMFBETA (Glia MatuRation Factor, Beta) ELISA Kit Packaging:96 wells Luciferase catalyzes the reaction: D-fireflyThe Human GMFBETA (Glia MatuRation Factor, Beta) ELISA Kit measures GMFB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Gmfb antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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