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CPE present in the sample is added and binds to antibodies coated on the wells
The concentration of Chicken IFNb in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human LTBP2 Antibody is added and binds to LTBP2 in the sample
And then biotinylated Mouse MMRN1 Antibody is added and binds to MMRN1 in the sample
Porcine AP17 (Apelin 17) ELISA Kit LD78ALPHA CPE present in the sampleThe Porcine AP17 (Apelin 17) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Porcine AP17. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Porcine AP17. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The
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