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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Irisin
Compared with the traditional boiling method
The concentration of Human HP in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve
EasyStep Human 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit Recombinant Standards or samples are addedThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with Human 25 OH D antigen, and the Human 25 OH D standard plate wells that pre coated using protein related techniques are provided separately. Standard Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP conjugated antibody specific to Human 25 OH D to each
