Substrate solution is then added and color develops in proportion to the amount of Human WNT7B
And then biotinylated Human LTBR Antibody is added and binds to LTBR in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated FH antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9
Rat CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1) ELISA Kit measures CYP24A1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CYP24A1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound