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Then a biotinylated detection antibody specific for Human SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated IL26 antibody
OC90 present in the sample is added and binds to antibodies coated on the wells
The concentration of Human GPC1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human PKP4 (Plakophilin 4) ELISA Kit Matrilysin Then a biotinylated detection antibodyThe Human (PKP4) Plakophilin 4 ELISA Kit measures Plakophilin 4 in samples. The plate has been pre coated with Human PKP4 antibody. PKP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PKP4 Antibody is added and binds to PKP4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PKP4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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