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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LEPR
GM present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Human MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
CCL3 present in the sample is added and binds to antibodies coated on the wells
Human NOTUM (Protein Notum Homolog) ELISA Kit TMVCF The microtiter plate provided inThe Human (NOTUM) Protein Notum Homolog ELISA Kit measures Protein notum homolog in samples. The plate has been pre coated with Human NOTUM antibody. NOTUM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NOTUM Antibody is added and binds to NOTUM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NOTUM antibody. After incubation unbound Streptavidin HRP is washed away
