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The concentration of Human MCP-1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated NRCAM antibody
You can calculate the concentration of Human CHEM in the samples by comparing the OD of the samples to the standard curve
only those wells that contain Mouse CLCF1
Rat LUM (Lumican) ELISA Kit Packaging:96 wells The concentration of Human MCP-1The Rat (LUM) Lumican ELISA Kit measures Lumican in Rat samples. The plate has been pre coated with Rat LUM antibody. LUM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LUM Antibody is added and binds to LUM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LUM antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution is
