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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD27BP
Substrate solution is then added and color develops in proportion to the amount of Mouse GDF5
The concentration of Human KRT12 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human LIMK1 Antibody is added and binds to LIMK1 in the sample
Mouse TSC2 (Tuberous Sclerosis Protein 2) ELISA Kit Packaging:96 wells Standards or samples are addedThe Mouse TSC2 (Tuberous Sclerosis Protein 2) ELISA Kit measures TSC2 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TSC2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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