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and the Human TIMP1 standard plate wells that pre-coated using protein-related techniques are provided separately
You can calculate the concentration of Human TIMP-1 in the samples by comparing the OD of the samples to the standard curve
then added a HRP-conjugated antibody specific to Rat PG to each microplate well and incubated
You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve
Mini-sample Mouse LEP (Leptin) ELISA Kit DNA/RNA Extraction Kits and the Human TIMP1 standardThis Leptin ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components
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