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The concentration of GCSF in the samples is then determined by comparing the OD of the samples to the standard curve
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 41 nm
Substrate solution is then added and color develops in proportion to the amount of Human C5AR1
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFNg
Human TA-IgG (Trophoblast antigen-Immunoglobulin G) ELISA Kit Packaging:96 wells The concentration of GCSF inThe Human TA IgG (Trophoblast antigen Immunoglobulin G) ELISA Kit measures TA IgG in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection antibody, then it binds