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Then a biotinylated detection antibody specific for Rat MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human NDRG1
The concentration of Rat EPO in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPG
Human MMP-26 (Matrix Metalloproteinase-26) ELISA Kit ADIPQTL1 Then a biotinylated detection antibodyThe Human (MMP 26) Matrix Metalloproteinase 26 ELISA Kit measures Matrix Metalloproteinase 26 in samples. The plate has been pre coated with Human MMP26 antibody. MMP26 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MMP26 Antibody is added and binds to MMP26 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MMP26 antibody. After incubation unbound Streptavidin HRP is
