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Rabbit Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol
ZG16B present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of UNC5B in the sample by plotting standard curve
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of A-GHR in the sample
Chicken RUNX2 (Runt-Related Transcription Factor 2) ELISA Kit Packaging:96 wells Rabbit Cortisol in samples (orThe Chicken RUNX2 (Runt Related Transcription Factor 2) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Chicken RUNX2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken RUNX2 and Avidin Horseradish Peroxidase (HRP) conjugate are added
