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Substrate solution is then added and color develops in proportion to the amount of Rat GZMB
The Pregnenolone ELISA is a competitive immunoassay
The concentration of Mouse LPAR3 in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human CEA
Rat NOX1 (Nadphoxidase 1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (NOX1) Nadphoxidase 1 ELISA Kit measures Nadph Oxidase 1 in Rat samples. The plate has been pre coated with Rat NOX1 antibody. NOX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NOX1 Antibody is added and binds to NOX1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NOX1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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