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You can calculate the concentration of Mouse αFP in the samples by comparing the OD of the samples to the standard curve
DYNC1H1 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Mouse SPP1
In the second incubation step
Human RELN (Reelin) ELISA Kit Packaging:96 wells You can calculate the concentrationThe Human (RELN) Reelin ELISA Kit measures Reelin in samples. The plate has been pre coated with Human RELN antibody. RELN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RELN Antibody is added and binds to RELN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RELN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
