Mouse Irisin ELISA Kit (Small Sample) KIM-1 only those wells that contain

only those wells that contain Human MFAP4

and the color intensity is directly proportional to the amount of Human CD55 present

The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Drosophila Melanogaster GAPDH Mouse Monoclonal Antibody (7B7)

Mouse Irisin ELISA Kit (Small Sample) KIM-1 only those wells that containThe Mouse Irisin ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse Irisin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse Irisin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only