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Universal FA/VB9 in the sample or standard competes with a fixed amount of Universal FA/VB9 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal FA/VB9
a capture antibody-IAPP-biotin-detection antibody complex was formed
The plate has been pre-coated with Rat IL2 antibody
You can calculate the concentration of Human KL in the samples by comparing the OD of the samples to the standard curve
Rabbit BFGF/FGF2 (Basic Fibroblast Growth Factor) QuickTest ELISA Kit Packaging:96 wells Universal FA/VB9 in the sampleThe Rabbit BFGF FGF2 (Basic Fibroblast Growth Factor) QuickTest ELISA Kit measures bFGF FGF2 in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards