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The concentration of Human APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM48
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCL6
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL3
Bovine CCL8 (C-C Motif Chemokine 8) ELISA Kit Packaging:96 wells The concentration of Human APLNThe Bovine (CCL8) C C Motif Chemokine 8 ELISA Kit measures C C motif chemokine 8 in Bovine samples. The plate has been pre coated with Bovine CCL8 antibody. CCL8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine CCL8 Antibody is added and binds to CCL8 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CCL8 antibody. After incubation unbound Streptavidin HRP is washed away
