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then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated
You can calculate the concentration of Porcine IL-1Alpha in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated CSK antibody
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLP1
Human IL-26 (Interleukin 26) ELISA Kit Packaging:96 wells then added a HRP-conjugated antibodyThe Human (IL 26) Interleukin 26 ELISA Kit measures Interleukin 26 in samples. The plate has been pre coated with Human IL26 antibody. IL26 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL26 Antibody is added and binds to IL26 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL26 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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