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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT2
And then biotinylated Mouse Ddit3 Antibody is added and binds to Ddit3 in the sample
You can calculate the concentration of Mouse TNF-Beta in the samples by comparing the OD of the samples to the standard curve
And then biotinylated Mouse CLEC18A Antibody is added and binds to CLEC18A in the sample
Human KRT2 (Keratin, Type Ii Cytoskeletal 2 Epidermal) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (KRT2) Keratin, Type Ii Cytoskeletal 2 Epidermal ELISA Kit measures Keratin, Type II Cytoskeletal 2 Epidermal in samples. The plate has been pre coated with Human KRT2 antibody. KRT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KRT2 Antibody is added and binds to KRT2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KRT2 antibody. After incubation unbound
