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The concentration of Mouse DEFb2 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM48
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human C5a are added to the micro ELISA plate wells
only those wells that contain Human GDF1
Human ELAVL1 (Elav-like Protein 1) ELISA Kit Packaging:96 wells The concentration of Mouse DEFb2The Human (ELAVL1) Elav like Protein 1 ELISA Kit measures ELAV like protein 1 in samples. The plate has been pre coated with Human ELAVL1 antibody. ELAVL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ELAVL1 Antibody is added and binds to ELAVL1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ELAVL1 antibody. After incubation unbound Streptavidin HRP is washed away
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