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Then Streptavidin-HRP is added and binds to the Biotinylated LCA antibody
Calculate the concentration of Alcam in the sample by plotting standard curve
Calculate the concentration of TNFRSF10B in the sample by plotting standard curve
Substrate solution is then added and color develops in proportion to the amount of Human HK2
Human GPC2 (Glypican 2) ELISA Kit ANF Then Streptavidin-HRP is added andThe Human GPC2 (Glypican 2) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human GPC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC2 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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