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EPHB1 present in the sample is added and binds to antibodies coated on the wells
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of CTCFL in the sample
it binds with BMP-7 bound to precoated antibody
And then biotinylated Human GAD1 Antibody is added and binds to GAD1 in the sample
Rat GCLC (Glutamate-cysteine ligase catalytic subunit) QuickTest ELISA Kit Packaging:96 wells EPHB1 present in the sampleThe Rat GCLC (Glutamate cysteine ligase catalytic subunit) QuickTest ELISA Kit measures GCLC in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and
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