Human IgD (Immunoglobulin D) ELISA Kit Packaging:96 wells only those wells that contain

only those wells that contain Mouse IL1R1

Substrate solution is then added and color develops in proportion to the amount of Human PDIA4

And then biotinylated Bovine CHI3L1 Antibody is added and binds to CHI3L1 in the sample

Then Streptavidin-HRP is added and binds to the Biotinylated RYR2 antibody

Human IgD (Immunoglobulin D) ELISA Kit Packaging:96 wells only those wells that containThe Human IgD (Immunoglobulin D) ELISA Kit measures IgD in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IgD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.