Human HEPH (Hephaestin) ELISA Kit Packaging:96 wells The plate has been pre-coated

The plate has been pre-coated with Rat HTR2B antibody

and the color intensity is directly proportional to the amount of Human Tie-1 present

Substrate solution is then added and color develops in proportion to the amount of Mouse ASGR2

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CCR2

Human HEPH (Hephaestin) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Human HEPH (Hephaestin) ELISA Kit measures HEPH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HEPH antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.