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The concentration of Human CGa in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Mouse C4 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The CD3 (Cluster of Differentiation 3) ELISA Kit is a Sandwich enzyme immunoassay
Bovine LGMN (Legumain) ELISA Kit Packaging:96 wells The concentration of Human CGaThe Bovine (LGMN) Legumain ELISA Kit measures Legumain in Bovine samples. The plate has been pre coated with Bovine LGMN antibody. LGMN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine LGMN Antibody is added and binds to LGMN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LGMN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
