Bovine DEFB1 (Beta-defensin 1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human DS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL12A

The concentration of Human HBg2 in the samples is then determined by comparing the OD of the samples to the standard curve

The OD value is proportional to the concentration of Mouse RANκL

Bovine DEFB1 (Beta-defensin 1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Bovine (DEFB1) Beta defensin 1 ELISA Kit measures Beta defensin 1 in Bovine samples. The plate has been pre coated with Bovine DEFB1 antibody. DEFB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine DEFB1 Antibody is added and binds to DEFB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated DEFB1 antibody. After incubation unbound Streptavidin HRP is washed away