Rat F9 (Fcoagulation Factor 9) ELISA Kit 42026 then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25

only those wells that contain Zebrafish IL2

Then Streptavidin-HRP is added and binds to the Biotinylated AFM antibody

And then biotinylated Human COPA Antibody is added and binds to COPA in the sample

Rat F9 (Fcoagulation Factor 9) ELISA Kit 42026 then added a HRP-conjugated antibodyThe Rat (F9) Fcoagulation Factor 9 ELISA Kit measures Fcoagulation Factor 9 in Rat samples. The plate has been pre coated with Rat F9 antibody. F9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat F9 Antibody is added and binds to F9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated F9 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.