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Substrate solution is then added and color develops in proportion to the amount of Human CASP7
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PP
Then a biotinylated detection antibody specific for Rat C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Bovine NPR3
Rat YWHAE (14-3-3 Protein Epsilon) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (YWHAE) 14 3 3 Protein Epsilon ELISA Kit measures 14 3 3 Protein Epsilon in Rat samples. The plate has been pre coated with Rat YWHAE antibody. YWHAE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat YWHAE Antibody is added and binds to YWHAE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated YWHAE antibody. After incubation unbound Streptavidin HRP is washed away
