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UACA in the sample or standard competes with a fixed amount of biotin-labeled UACA for sites on a pre-coated antibody specific to UACA
The OD value is proportional to the concentration of Rabbit COL10
Calculate the concentration of FGF10 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated DKK1 antibody
Guinea pig GDF9 (Growth Differentiation Factor 9) ELISA Kit MS5 UACA in the sample orThe Guinea pig GDF9 (Growth Differentiation Factor 9) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig GDF9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig GDF9 and Avidin Horseradish Peroxidase (HRP) conjugate are added
