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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BRAK
Then a biotinylated detection antibody specific for Rat PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated IFIH1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human STX4
Mouse BDNF (Brain Derived Neurotrophic Factor) ELISA Kit Packaging:96 wells Standards or samples are addedThe Mouse BDNF (Brain Derived Neurotrophic Factor) ELISA Kit measures BDNF in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BDNF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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