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Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Mouse POU5F1
This Zonulin ELISA kit uses the Sandwich-ELISA principle
it binds with DDOST bound to precoated antibody
Mouse LRP6 (Low-density lipoprotein receptor-related protein 6) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse LRP6 (Low density lipoprotein receptor related protein 6) ELISA Kit measures Lrp6 in Mouse Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Lrp6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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