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Then a biotinylated detection antibody specific for Porcine IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
PYCRL present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Mouse CASP3
Calculate the concentration of TESPA1 in the sample by plotting standard curve
Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat CNTF (Ciliary Neurotrophic Factor) ELISA Kit measures CNTF in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CNTF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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