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And then biotinylated Rat GPx-4 Antibody is added and binds to GPx-4 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated CUTA antibody
Only those wells that contain Human AGC
The concentration of Dog IL1b in the samples is then determined by comparing the OD of the samples to the standard curve
Sheep IL-6 (Interleukin 6) ELISA Kit Packaging:96 wells And then biotinylated Rat GPx-4The Sheep (IL 6) Interleukin 6 ELISA Kit measures Interleukin 6 in Sheep samples. The plate has been pre coated with Sheep IL 6 antibody. IL 6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep IL 6 Antibody is added and binds to IL 6 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL 6 antibody. After incubation unbound Streptavidin HRP is washed away during a washing