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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VAP-1
Substrate solution is then added and color develops in proportion to the amount of Rat AGER
The plate has been pre-coated with Mouse LTB4R2 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated CTF2 antibody
Human PDE4A (cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit Lymphotoxin Alpha Standards or samples are addedThe Human PDE4A (cAMP specific 3',5' cyclic phosphodiesterase 4A) ELISA Kit measures PDE4A in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PDE4A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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