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only those wells that contain Cattle IL12A
The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve
the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of leptin present
only those wells that contain Rabbit EGF
Mouse LIF (Leukemia Inhibitory Factor) ELISA Kit (Mini-Sample) endocan only those wells that containThis Mouse LIF (Leukemia Inhibitory Factor) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to LIF . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LIF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
