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The plate has been pre-coated with Human GALNT11 antibody
And then biotinylated Human IFNA21 Antibody is added and binds to IFNA21 in the sample
a horseradish peroxidase conjugated mouse anti-Human IL-12 Antibody is added to the wells and binds to IL-12 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S1PR5
Mouse SFRP1 (Secreted Frizzled Related Protein 1) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Mouse (SFRP1) Secreted Frizzled Related Protein 1 ELISA Kit measures Secreted Frizzled Related Protein 1 in Mouse samples. The plate has been pre coated with Mouse SFRP1 antibody. SFRP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SFRP1 Antibody is added and binds to SFRP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SFRP1 antibody. After incubation unbound
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