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The concentration of Human sICAM1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NRN1
Then Streptavidin-HRP is added and binds to the Biotinylated COX7A1 antibody
You can calculate the concentration of VEGF165 in the samples by comparing the OD of the samples to the standard curve
Rat KRT19 (Keratin 19) ELISA Kit Packaging:96 wells The concentration of Human sICAM1The Rat (KRT19) Keratin 19 ELISA Kit measures Keratin 19 in Rat samples. The plate has been pre coated with Rat KRT19 antibody. KRT19 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat KRT19 Antibody is added and binds to KRT19 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KRT19 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
