PCNA Mouse mAb MDA7 PCR and other inhibitors of

PCR and other inhibitors of enzymatic reaction could be effectively removed by two-step washing steps

coli expression system and the target gene encoding Ala2-Asp135 is expressed with a 6His tag at the C-terminus

and the supernatant layer is collected

You can calculate the concentration of Human SEPP1 in the samples by comparing the OD of the samples to the standard curve

PCNA Mouse mAb MDA7 PCR and other inhibitors ofPCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes