Human TFF3 (Trefoil Factor 3) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSPAN1

You can calculate the concentration of Mouse MPIF2 in the samples by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Mouse OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

You can calculate the concentration of Rat Irisin in the samples by comparing the OD of the samples to the standard curve

Human TFF3 (Trefoil Factor 3) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (TFF3) Trefoil Factor 3 ELISA Kit measures Trefoil Factor 3 in samples. The plate has been pre coated with Human TFF3 antibody. TFF3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TFF3 Antibody is added and binds to TFF3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TFF3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing