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The OD value is proportional to the concentration of Human C3a
You can calculate the concentration of Mouse Klotho in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human Cyr61 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A
Guinea Pig IFN-gamma ELISA Kit Packaging:48 wells The OD value is proportionalThe Guinea Pig IFN gamma ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Guinea pig IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Guinea pig IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
