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When the sample containing MMP-8 is added to the plate
a horseradish peroxidase conjugated mouse anti-Human PHPT1 Antibody is added to the wells and binds to PHPT1 in the sample
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BRAK
Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human CASP7 (Caspase 7) ELISA Kit GP When the sample containing MMP-8The Human (CASP7) Caspase 7 ELISA Kit measures Caspase 7 in samples. The plate has been pre coated with Human CASP7 antibody. CASP7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CASP7 Antibody is added and binds to CASP7 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CASP7 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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